Anti-PCGF6 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9BYE7
Gene Id	84108
Gene Symbols	PCGF6
Protein Name / Synonyms	PCGF6, MBLR, RNF134, polycomb group ring finger 6
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

30kDa/39kDa

Observed MW

39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 161-350 of human PCGF6 (NP_001011663.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of MCF7 cells, using PCGF6 antibody (144-05760) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 120s.
Western blot - PCGF6 Polyclonal Antibody (144-05760)

Immunofluorescence analysis of U2OS cells using PCGF6 antibody (144-05760).
Immunofluorescence - PCGF6 Polyclonal Antibody (144-05760)

Expiration:

12 months from the date of shipment when stored properly.

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