Anti-PCMT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P22061
Gene Id	5110
Gene Symbols	PCMT1
Protein Name / Synonyms	PCMT1, PIMT, protein-L-isoaspartate(D-aspartate) O-methyltransferase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

24kDa

Observed MW

25kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PCMT1 antibody (144-06684).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - PCMT1 antibody (144-06684)

Immunofluorescence analysis of HeLa cells using PCMT1 antibody (144-06684). Blue: DAPI for nuclear staining.
Immunofluorescence - PCMT1 antibody (144-06684)

Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug PCMT1 antibody (144-06684). Western blot was performed from the immunoprecipitate using PCMT1 antibody (144-06684) at a dilition of 1:1000.
Immunoprecipitation - 1ug PCMT1 antibody (144-06684)

Expiration:

12 months from the date of shipment when stored properly.

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