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ISO 13485:2016 cGMP
Anti-PD-1 Antibody
Catalog #: 130-10749
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | Q15116 |
| Gene Id | 5133 |
| Gene Symbols | PDCD1 PD1 |
| Protein Name / Synonyms | PD-1 |
| Clonality | Monoclonal |
| Clone | 2H7-F7-E8 |
| Immunogen | Immunogen was Recombinant protein Human PD-1. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution = 1:320, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in 1X PBS, aliquot using ddH2O. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cancer, Autoimmune Disease, Cancer Immunology, Adaptive Immunity |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Expiration
12 months from the date of shipment when stored properly.
Flow Cytometry Image
PHA stimulated (3 days) human PBMCs were stained with either Purified Mouse Anti-Human PD-1 primary antibody (#130-10749, blue histogram), or mouse IgG isotype control (gray histogram), followed by anti-mouse IgG/IgM RayBright R 647 secondary antibody.
Western Blot Analysis
Secondary Antibody: HRP Goat Anti-Mouse IgG (H + L) 1:5000
Description
Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells.
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.