PDCD4 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q53EL6 |
| Gene Id | 27250 |
| Gene Symbols | PDCD4 |
| Protein Name / Synonyms | PDCD4, H731, programmed cell death 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
50kDa/51kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PDCD4 antibody (144-02570) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.![Western blot - [KO Validated] PDCD4 Polyclonal Antibody (144-02570)](https://cdn.sanity.io/images/g51potcz/production/3f960afc3ebc88bd4e8373cfaf3762c42e1495ad-378x350.jpg)
Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) HeLa cells, using PDCD4 antibody (144-02570) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] PDCD4 Polyclonal Antibody (144-02570)](https://www.raybiotech.com/files/images/Antibodies/144/144-02570/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.