Anti-PDCD6 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O75340
Gene Id	10016
Gene Symbols	PDCD6
Protein Name / Synonyms	PDCD6, ALG-2, ALG2, PEF1B, programmed cell death 6
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

7kDa/21kDa

Observed MW

16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human PDCD6 (NP_037364.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PDCD6 antibody (144-06685) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PDCD6 Polyclonal Antibody (144-06685)

Immunohistochemistry of paraffin-embedded rat kidney using PDCD6 antibody (144-06685) at dilution of 1:200 (40x lens).
Immunohistochemistry - PDCD6 Polyclonal Antibody (144-06685)

Immunofluorescence analysis of HeLa cells using PDCD6 antibody (144-06685). Blue: DAPI for nuclear staining.
Immunofluorescence - PDCD6 Polyclonal Antibody (144-06685)

Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug PDCD6 antibody (144-06685). Western blot was performed from the immunoprecipitate using PDCD6 antibody (144-06685) at a dilition of 1:1000.
Immunoprecipitation - PDCD6 Polyclonal Antibody (144-06685)

Expiration:

12 months from the date of shipment when stored properly.

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