Anti-PDE4D Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	Q08499
Gene Id	5144
Gene Symbols	PDE4D
Protein Name / Synonyms	PDE4D, ACRDYS2, DPDE3, HSPDE4D, PDE43, PDE4DN2, STRK1, phosphodiesterase 4D
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.

Dilutions

WB 1:500 - 1:2000
IF 1:20 - 1:100

Format

Calculated MW

23-24kDa/57-91kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 249-518 of human PDE4D (NP_001184152.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PDE4D antibody (144-60527) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PDE4D Polyclonal Antibody (144-60527)

Immunofluorescence analysis of HeLa cells using PDE4D antibody (144-60527). Blue: DAPI for nuclear staining.
Immunofluorescence - PDE4D Polyclonal Antibody (144-60527)

Expiration:

12 months from the date of shipment when stored properly.

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