Anti-PDIA6 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q15084
Gene Id	10130
Gene Symbols	PDIA6
Protein Name / Synonyms	PDIA6, ERP5, P5, TXNDC7, protein disulfide-isomerase A6
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

47kDa/48kDa/53kDa

Observed MW

48kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human PDIA6 (NP_005733.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PDIA6 antibody (144-07055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - PDIA6 antibody (144-07055)

Immunohistochemistry of paraffin-embedded rat brain using PDIA6 Antibody (144-07055) at dilution of 1:100 (40x lens).
Immunohistochemistry - PDIA6 Antibody (144-07055)

Immunohistochemistry of paraffin-embedded mouse brain using PDIA6 Antibody (144-07055) at dilution of 1:100 (40x lens).
Immunohistochemistry - PDIA6 Antibody (144-07055)

Immunofluorescence analysis of U2OS cells using PDIA6 antibody (144-07055). Blue: DAPI for nuclear staining.
Immunofluorescence - PDIA6 antibody (144-07055)

Expiration:

12 months from the date of shipment when stored properly.