Anti-PDLIM5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q96HC4
Gene Id	10611
Gene Symbols	PDLIM5
Protein Name / Synonyms	PDLIM5, ENH, ENH1, L9, LIM, PDZ and LIM domain 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

15kDa/23kDa/25kDa/52kDa/53kDa/63kDa/67kDa

Observed MW

68kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 317-596 of human PDLIM5 (NP_006448.4).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PDLIM5 antibody (144-05720) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PDLIM5 Polyclonal Antibody (144-05720)

Immunofluorescence analysis of U2OS cells using PDLIM5 antibody (144-05720). Blue: DAPI for nuclear staining.
Immunofluorescence - PDLIM5 Polyclonal Antibody (144-05720)

Expiration:

12 months from the date of shipment when stored properly.

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