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Anti-PDX1 (C-term) Antibody
Catalog #: 102-14683
Rabbit Anti-Human PDX1 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O00330 |
| Gene Id | 8050 |
| Gene Symbols | PDHX |
| Protein Name / Synonyms | PDX1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-459 amino acids from the C-terminal region of human PDX1. |
| Concentration (lot specific) | 1.600 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
Specificity
Other Names
PDHX; PDX1; Pyruvate dehydrogenase protein X component, mitochondrial; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3-binding protein; Lipoyl-containing pyruvate dehydrogenase complex component X; proX
NCBI Accession #
NP_001128496.1;NP_001159630.1;NP_003468.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
54122
Recommended Dilutions
WB: 1:1000
 | Western blot analysis of lysate from WiDr cell line, using hPDX1R444(Cat# 102-14683). 102-14683 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. |
 | Western blot analysis of lysate from WiDr cell line, using hPDX1R444(Cat# 102-14683). 102-14683 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.