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Anti-PER1 Antibody
Catalog #: 144-08449
PER1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O15534 |
| Gene Id | 5187 |
| Gene Symbols | PER1 |
| Protein Name / Synonyms | PER1, PER, RIGUI, hPER, period circadian clock 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
Format
Calculated MW
91kDa/136kDa
Observed MW
160kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PER1 (NP_002607.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PER1 antibody (144-08449) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using PER1 antibody (144-08449) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PER1 antibody (144-08449) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PER1 antibody (144-08449) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PER1 antibody (144-08449) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.