3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-PEX14 Antibody
Catalog #: 144-07336
PEX14 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O75381 |
| Gene Id | 5195 |
| Gene Symbols | PEX14 |
| Protein Name / Synonyms | PEX14, NAPP2, PBD13A, Pex14p, dJ734G22.2, peroxisomal biogenesis factor 14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
36kDa/41kDa
Observed MW
41kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse liver, using PEX14 antibody (144-07336) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate cancer using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PEX14 antibody (144-07336). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.