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Anti-PFKM Antibody
Catalog #: 144-05477
PFKM Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P08237 |
| Gene Id | 5213 |
| Gene Symbols | PFKM |
| Protein Name / Synonyms | PFKM, ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKX, PPP1R122, phosphofructokinase, muscle |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
81kDa/85kDa/93kDa
Observed MW
85kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human PFKM (NP_001160160.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PFKM antibody (144-05477) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using PFKM antibody (144-05477) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PFKM antibody (144-05477) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.