Anti-PFKP (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q01813
Gene Id	5214
Gene Symbols	PFKP
Protein Name / Synonyms	PFKP (C-term)
Clonality	Polyclonal
Preparation	This PFKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-784 amino acids from the C-terminal region of human PFKP.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Specificity

Other Names

PFKP; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; Phosphofructo-1-kinase isozyme C; Phosphofructokinase 1; Phosphohexokinase

NCBI Accession #

NP_001229268.1;NP_002618.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

85596

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	PFKP Antibody (D769) (Cat# 102-17815) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PFKP antibody detected the PFKP protein (arrow).
	Fibroblasts were cultured for 2 d in DMEM supplemented with 10% TH-depleted bovine serum. They were then treated with 2 nM T3 in the absence or presence of either LY294002 (LY, 50 M) or PD98059 (PD, 50 M), added 1 h before the addition of T3. Cells were harvested 24 h after T3 treatment. Whole-cell lysates, obtained from two separately treated culture dishes for each treatment, were subjected to WB with anti-PFKP. (Mol. Endocrinol. 2005 Dec 01;19(12):2955-2963)
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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