RayBiotech

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ISO 13485:2016 cGMP

Anti-PFN2 Antibody

Catalog #: 144-03074

PFN2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P35080
Gene Id
5217
Gene Symbols
PFN2
Protein Name / Synonyms
PFN2, D3S1319E, PFL, profilin-2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
15kDa
Observed MW
15kDa

Immunogen

A synthetic peptide of human PFN2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PFN2 antibody (144-03074) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - PFN2 antibody (144-03074)

Expiration:
12 months from the date of shipment when stored properly.