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Anti-PGAM1 Antibody
Catalog #: 144-04015
PGAM1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P18669 |
| Gene Id | 5223 |
| Gene Symbols | PGAM1 |
| Protein Name / Synonyms | PGAM1, HEL-S-35, PGAM-B, PGAMA, phosphoglycerate mutase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:2000
Format
Calculated MW
28kDa
Observed MW
29kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human PGAM1 (NP_002620.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PGAM1 antibody (144-04015) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.