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Anti-PHC3 Antibody
Catalog #: 144-06479
PHC3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8NDX5 |
| Gene Id | 80012 |
| Gene Symbols | PHC3 |
| Protein Name / Synonyms | PHC3, EDR3, HPH3, polyhomeotic homolog 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
14kDa/16kDa/19kDa/35kDa/104kDa/106kDa/107kDa
Observed MW
106kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human PHC3 (NP_079223.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PHC3 Antibody (144-06479) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using PHC3 antibody (144-06479). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.