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Anti-PHF1 Antibody
Catalog #: 144-08450
PHF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43189 |
| Gene Id | 5252 |
| Gene Symbols | PHF1 |
| Protein Name / Synonyms | PHF1, MTF2L2, PCL1, PHF2, TDRD19C, hPHF1, PHD finger protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
49kDa/62kDa
Observed MW
62kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PHF1 (NP_077084.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PHF1 antibody (144-08450) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using PHF1 antibody (144-08450) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using PHF1 antibody (144-08450) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PHF1 antibody (144-08450) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PHF1 antibody (144-08450) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.