Anti-PHF11 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UIL8
Gene Id	51131
Gene Symbols	PHF11
Protein Name / Synonyms	PHF11, APY, BCAP, IGEL, IGER, IGHER, NY-REN-34, NYREN34, PHD finger protein 11
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

33kDa/37kDa

Observed MW

34kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human PHF11 (NP_001035533.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PHF11 antibody (144-07803) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PHF11 antibody (144-07803)

Immunofluorescence analysis of A549 cells using PHF11 antibody (144-07803). Blue: DAPI for nuclear staining.
Immunofluorescence - PHF11 antibody (144-07803)

Expiration:

12 months from the date of shipment when stored properly.

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