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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q5T6S3 |
| Gene Id | 26147 |
| Gene Symbols | PHF19 |
| Protein Name / Synonyms | PHF19, MTF2L1, PCL3, TDRD19B, PHD finger protein 19 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
14kDa/22kDa/65kDa
Observed MW
66kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human PHF19 (NP_001009936.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PHF19 antibody (144-08065) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human oophoroma using PHF19 antibody (144-08065) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using PHF19 antibody (144-08065).
Expiration:
12 months from the date of shipment when stored properly.