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Anti-PHKA1 (C-term) Antibody
Catalog #: 107-10839
PHKA1 antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 5255 |
| Protein Name / Synonyms | PHKA1, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
MGC132604 antibody, PHKA antibody, PHKA1 antibody, "phosphorylase kinase, alpha 1 (muscle), muscle glycogenosis antibody", phosphorylase b kinase regulatory subunit alpha skeletal muscle isoform antibody, "phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform antibody", phosphorylase kinase alpha M subunit antibody, "phosphorylase kinase, alpha 1 (muscle) antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
137 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 828 and 1062 of PHKA1 (Uniprot ID# P46020)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using PHKA1(107-10839) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PHKA1(107-10839) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
107-10839 diluted at 1:1000
A: HeLa
7.5% SDS PAGE
107-10839 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.