Anti-Phospho-AKT1-S473 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P31749
Gene Id	207
Gene Symbols	AKT1
Protein Name / Synonyms	AKT1, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT serine/threonine kinase 1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Apoptosis, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Dilutions

WB 1:500 - 1:2000; IHC 1:50 - 1:200

Format

Calculated MW

48kDa/55kDa

Observed MW

56kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S473 of human AKT1

Modification

Phospho S473

Storage

Store at -20°. Avoid freeze / thaw cycles.

Images

Western blot

Expiration:

12 months from the date of shipment when stored properly.

Featured Products