Anti-Phospho-Bcl2 -S70 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	P10415
Gene Id	596
Gene Symbols	BCL2
Protein Name / Synonyms	BCL2, Bcl-2, PPP1R50, apoptosis regulator Bcl-2
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Apoptosis, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

22kDa/26kDa

Observed MW

26kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S70 of human Bcl2

Modification

Phospho S70

Storage

-20°C

Images

Western blot analysis of extracts of Jurkat and 293 cells, using Phospho-Bcl2 -S70 antibody (144-50575) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-BCL2-S70 pAb (144-50575)

Immunofluorescence analysis of HeLa cells using BCL2 antibody (144-50575) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-BCL2-S70 pAb (144-50575)

Expiration:

12 months from the date of shipment when stored properly.

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