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Anti-Phospho-CHEK1-S280 Antibody
Catalog #: 144-50625
Phospho-CHEK1-S280 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O14757 |
| Gene Id | 1111 |
| Gene Symbols | CHEK1 |
| Protein Name / Synonyms | CHEK1, CHK1, checkpoint kinase 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Format
Calculated MW
43kDa/50kDa/54kDa
Observed MW
56kDa
Immunogen
A synthetic phosphorylated peptide around S280 of human CHEK1 (NP_001265.2).
Modification
Phospho S280
Storage
Store at -20°. Avoid freeze / thaw cycles.
Images
Expiration:
12 months from the date of shipment when stored properly.