Anti-Phospho-CHK1 (S317) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O14757
Gene Id	1111
Gene Symbols	CHEK1
Protein Name / Synonyms	Phospho-CHK1(S317)
Clonality	Polyclonal
Preparation	This CHK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S317 of human CHK1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Dot Blotting, Western Blotting, IHC-P
Research Area	DNA Damage
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.

Specificity

Other Names

CHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1

NCBI Accession #

NP_001107593.1;NP_001107594.1;NP_001231775.1;NP_001265.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

54434

Recommended Dilutions

DB: 1:500; WB: 1:1000; IHC: 1:50-100

	Dot blot analysis of anti-Phospho-CHK1-S317 Antibody (Cat# 102-15291) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.
	The anti-Phospho-CHK1-S317 Pab (Cat# 102-17191) is used in Western blot for detection in, from left to right, Jurkat, A2058, Hela, and HL60 tissue lysates.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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