Anti-Phospho-eNos (S1177) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P29474
Gene Id	4846
Gene Symbols	NOS3
Protein Name / Synonyms	Phospho-eNos(S1177)
Clonality	Polyclonal
Preparation	This eNos Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1177 of human eNos.
Concentration (lot specific)	0.290 mg/ml
Recommended Applications	IF, Dot Blotting
Research Area	Angiogenesis, Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

NOS3; Nitric oxide synthase, endothelial; Constitutive NOS; EC-NOS; Endothelial NOS; NOS type III

NCBI Accession #

NP_000594.2;NP_001153581.1;NP_001153582.1;NP_001153583.1

Other Accession #

Q62600; Q28969; P70313; P29473

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

133289

Recommended Dilutions

DB: 1:500; IF: 1:10-50

	Dot blot analysis of anti-Phospho-eNos-S1177 Phospho-specific Pab (Cat# 102-15510) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Confocal immunofluorescent analysis of Phospho-eNos-S1177 Antibody (Cat# 102-15510) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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