Anti-Phospho-EP300 (S89) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q09472
Gene Id	2033
Gene Symbols	EP300
Protein Name / Synonyms	Phospho-EP300(S89)
Clonality	Polyclonal
Preparation	This EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	Dot Blotting, IHC-P
Research Area	Cardiovascular Disease, Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

Specificity

Predicted Reactivity

Mouse

Other Names

EP300; P300; Histone acetyltransferase p300; E1A-associated protein p300

NCBI Accession #

NP_001420.2

Other Accession #

B2RWS6

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

264161

Recommended Dilutions

DB: 1:500; IHC: 1:50-100

	Dot blot analysis of anti-Phospho-EP300-S89 Pab (Cat# 102-15333) on nitrocellulose membrane. 50ng of Phospho-peptide (BP3197a) or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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