Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P27361\|P28482
Gene Id	5594\|5595
Gene Symbols	MAPK1,MAPK3
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100

Format

Calculated MW

36kDa/41kDa/38kDa/40kDa/43kDa

Observed MW

38kDa, 41kDa

Immunogen

A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).

Modification

Phospho T202/Y204 + T185/Y187

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (144-50472) at 1:5000 dilution or ERK1/2 antibody (144-613146).293T cells and C6 cells were treated by ATP(5 mM) at 30? for 1 hour. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (144-50472)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (144-50472) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (144-50472)

Expiration:

12 months from the date of shipment when stored properly.

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