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Anti-Phospho-FOXO1-S319 Antibody
Catalog #: 144-50872
Phospho-FOXO1-S319 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P04049 |
| Gene Id | 2308 |
| Gene Symbols | RAF1 |
| Protein Name / Synonyms | NS5, CRAF, Raf-1, c-Raf, CMD1NN |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Dilutions
WB 1:500 - 1:2000; IP 1:50 - 1:100
Format
Calculated MW
73kDa
Observed MW
Immunogen
A synthetic phosphorylated peptide around S296 of human RAF1.
Modification
Storage
Store at -20°. Avoid freeze / thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.