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Anti-Phospho-FYN-S21 Antibody

Catalog #: 144-50510

Phospho-FYN-S21 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P06241
Gene Id
2534
Gene Symbols
FYN
Protein Name / Synonyms
FYN, SLK, SYN, p59-FYN, tyrosine-protein kinase Fyn
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaAngiogenesis, Cardiovascular Disease, Inflammation
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
54kDa/60kDa
Observed MW
61kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S21 of human FYN

Modification

Phospho S21

Storage

-20°C

Images

Western blot analysis of extracts of A-431 cells, using Phospho-FYN-S21 antibody (144-50510) at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-FYN-S21 antibody (144-50510)

Expiration:
12 months from the date of shipment when stored properly.