Anti-Phospho-GSK3A-S21 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P49840
Gene Id	2931
Gene Symbols	GSK3A
Protein Name / Synonyms	GSK3A, glycogen synthase kinase-3 alpha
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

50kDa

Observed MW

51kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S21 of human GSK3A

Modification

Phospho S21

Storage

-20°C

Images

Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3A-S21 antibody (144-50582) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-GSK3A-S21 pAb (144-50582)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3A-S21 pAb (144-50582). Western blot was performed from the immunoprecipitate using Phospho-GSK3A-S21 pAb (144-50582) at a dilition of 1:1000. 293T cells were treated by Insulin (100 nM) at 37? for 10 minutes after serum-starvation overnight.
Immunoprecipitation - Phospho-GSK3A-S21 pAb (144-50582)

Expiration:

12 months from the date of shipment when stored properly.

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