Anti-Phospho-HER2/ERBB2-Y1221/1222 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04626
Gene Id	2064
Gene Symbols	ERBB2
Protein Name / Synonyms	ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, MLN19, erb-b2 receptor tyrosine kinase 2
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis, Cardiovascular Disease, Cancer, HER/ErbB Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Dilutions

WB 1:500 - 1:1000

Format

Calculated MW

62kDa/70kDa/97kDa/134kDa/136kDa/137kDa

Observed MW

190kDa

Immunogen

A synthetic phosphorylated peptide around Y1221 & Y1222 of human HER2/ERBB2 (NP_004439.2).

Modification

Phospho Y1221/1222

Storage

Store at -20°. Avoid freeze / thaw cycles.

Images

Western blot

Expiration:

12 months from the date of shipment when stored properly.

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