Anti-Phospho-HSPB1-S15 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04792
Gene Id	3315
Gene Symbols	HSPB1
Protein Name / Synonyms	HSPB1, CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, heat shock protein beta-1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Research Area	Angiogenesis, Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

22kDa

Observed MW

28kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S15 of human HSPB1

Modification

Phospho S15

Storage

-20°C

Images

Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S15 antibody (144-50040) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5min.
Western blot - Phospho-HSPB1-S15 pAb (144-50040)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-HSPB1-S15 pAb (144-50040). Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S15 pAb (144-50040) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/ml) at 37? for 30 minutes after serum-starvation overnight.
Immunoprecipitation - Phospho-HSPB1-S15 pAb (144-50040)

Expiration:

12 months from the date of shipment when stored properly.

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