Anti-Phospho-LC3C (S12) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9H492
Gene Id	84557
Gene Symbols	MAP1LC3A
Protein Name / Synonyms	Phospho-LC3C(S12)
Clonality	Polyclonal
Preparation	This LC3C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human LC3C.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, Dot Blotting
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

MAP1LC3A; Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain 3 alpha

NCBI Accession #

NP_115903.1;NP_852610.1

Other Accession #

Q6XVN8; Q91VR7; Q2HJ23

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14272

Recommended Dilutions

WB: 1:1000; DB: 1:500

	Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa
	Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody (Cat# 102-15375) and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with 102-15375. The data shows that treatment with rapamycin showed no significant change in level of LC3.
	Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with 102-15375. The data shows that treatment with MPP+ showed no significant change in level of LC3.
	Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h.