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Anti-Phospho-MAP2K1-S298 Antibody
Catalog #: 144-50063
Phospho-MAP2K1-S298 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q02750 |
| Gene Id | 5604 |
| Gene Symbols | MAP2K1 |
| Protein Name / Synonyms | MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
40kDa/43kDa
Observed MW
43kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S298 of human MAP2K1
Modification
Phospho S298
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (144-50063) at 1:1000 dilution or MAP2K1 antibody (144-12687). HeLa cells were treated by PMA/TPA (200 nM) at 37? for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (144-50063) at 1:1000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37? for 1 hour or treated by ATP(5 mM) at 30? for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37? for 1 hour or treated by ATP(5 mM) at 30? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-MAP2K1-S298 pAb (144-50063). Western blot was performed from the immunoprecipitate using Phospho-MAP2K1-S298 pAb (144-50063) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37? for 30 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.