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Anti-Phospho-MAP2K1-T286 Antibody
Catalog #: 144-50065
Phospho-MAP2K1-T286 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q02750 |
| Gene Id | 5604 |
| Gene Symbols | MAP2K1 |
| Protein Name / Synonyms | MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
40kDa/43kDa
Observed MW
43kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding T286 of human MAP2K1
Modification
Phospho T286
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-T286 antibody (144-50065) at 1:2000 dilution or MAP2K1 antibody (144-12687). HeLa cells were treated by nocodazole (50 ng/ml) at 37? for 20 hours or treated by ATP(5 mM) at 30? for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37? for 20 hours or treated by ATP(5 mM) at 30? for 1 hour. PC-12 cells were treated by Nocodazole (1 ?g/ml) at 37? for 16 hours or treated by ATP(5 mM) at 30? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.