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Anti-Phospho-MAP2K4-S257/T261 Antibody
Catalog #: 144-50541
Phospho-MAP2K4-S257/T261 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P45985 |
| Gene Id | 6416 |
| Gene Symbols | MAP2K4 |
| Protein Name / Synonyms | MAP2K4, JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, mitogen-activated protein kinase kinase 4 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
44kDa/45kDa
Observed MW
44kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S257/T261 of human MAP2K4
Modification
Phospho S257/T261
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-MAP2K4-S257/T261 antibody (144-50541) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes or treated by ATP (5mM) at 30? for 1 hour .NIH/3T3 cells were treated by UV for 15-30 minutes or treated by ATP (5mM) at 30? for 1 hour .C6 cells were treated by UV for 15-30 minutes or treated by ATP (5mM) at 30? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.