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Anti-Phospho-MAP3K5-S83 Antibody
Catalog #: 144-50638
Phospho-MAP3K5-S83 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q99683 |
| Gene Id | 4217 |
| Gene Symbols | MAP3K5 |
| Protein Name / Synonyms | MAP3K5, ASK1, MAPKKK5, MEKK5, mitogen-activated protein kinase kinase kinase 5 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
69kDa/154kDa
Observed MW
155kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S83 of human MAP3K5
Modification
Phospho S83
Storage
Store at -20°. Avoid freeze / thaw cycles.
Images
Expiration:
12 months from the date of shipment when stored properly.