Anti-Phospho-MAP3K7-S412 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O43318
Gene Id	6885
Gene Symbols	MAP3K7
Protein Name / Synonyms	MAP3K7, CSCF, FMD2, MEKK7, TAK1, TGF1a, mitogen-activated protein kinase kinase kinase 7
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

53kDa/56kDa/64kDa/67kDa

Observed MW

67kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S412 of human MAP3K7

Modification

Phospho S412

Storage

-20°C

Images

Western blot analysis of extracts of K-562 cells, using Phospho-MAP3K7-S412 antibody (144-50071) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight or treated by PMA/TPA (200nM).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-MAP3K7-S412 antibody (144-50071)

Expiration:

12 months from the date of shipment when stored properly.

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