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Anti-Phospho-MAX-S11 Antibody
Catalog #: 144-50072
Phospho-MAX-S11 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P61244 |
| Gene Id | 4149 |
| Gene Symbols | MAX |
| Protein Name / Synonyms | MAX, bHLHd4, protein max |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
9kDa/11kDa/12kDa/15kDa/17kDa/18kDa
Observed MW
18kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S11 of human MAX
Modification
Phospho S11
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells, using Phospho-MAX-S11 antibody (144-50072) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Immunofluorescence analysis of U2OS cells using Phospho-MAX-S11 antibody (144-50072). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.