Anti-Phospho-MEF2C-S396 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q06413
Gene Id	4208
Gene Symbols	MEF2C
Protein Name / Synonyms	MEF2C, C5DELq14.3, DEL5q14.3, myocyte enhancer factor 2C
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

44kDa/47kDa/50kDa/51kDa/52kDa

Observed MW

60kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S396 of human MEF2C

Modification

Phospho S396

Storage

-20°C

Images

Western blot analysis of extracts of K-562 and MCF-7 cells, using Phospho-MEF2C-S396 antibody (144-50075) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. MCF7 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-MEF2C-S396 antibody (144-50075)

Immunofluorescence analysis of U2OS cells using Phospho-MEF2C-S396 antibody (144-50075). Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-MEF2C-S396 antibody (144-50075)

Expiration:

12 months from the date of shipment when stored properly.

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