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Anti-Phospho-MTOR-S2448 Antibody

Catalog #: 144-50094

Phospho-MTOR-S2448 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P42345
Gene Id
2475
Gene Symbols
MTOR
Protein Name / Synonyms
MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS, mechanistic target of rapamycin
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.05% proclin300, 50% glycerol, pH 7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCardiovascular Disease, Inflammation, mTOR Signaling, Autophagy
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
288kDa
Observed MW
289kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding serine 2448 of human mTOR/FRAP

Modification

Phospho S2448

Storage

-20°C

Images

Western blot analysis of extracts of NIH/3T3 and C6 cells, using Phospho-MTOR-S2448 antibody (144-50094) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. C6 cells were treated by IGF-1 (50 ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30S.
Western blot - Phospho-mTOR-S2448 pAb (144-50094)

Immunofluorescence analysis of HeLa cells using Phospho-MTOR-S2448 antibody (144-50094) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-mTOR-S2448 pAb (144-50094)

Expiration:
12 months from the date of shipment when stored properly.