Anti-Phospho NF-kB p65 (Ser536) (531~541aa ) Antibody

Product Description

Specifications

Size	20 µg, 100 µg, 200 µg, 500 µg, 1 mg
Estimated Lead Time	1-2 business days
Species	Human
Host Species	Rabbit
Accession Number	Q04206
Gene Id	5970
Gene Symbols	RELA NFKB3
Protein Name / Synonyms	Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
Clonality	Polyclonal
Preparation	This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen Phospho NF-kB p65 (Ser536) (531~541aa). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography.
Recommended Applications	ELISA (recommended work dilution= 1:5, 000)
Reconstitution	Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Research Area	Angiogenesis, Cancer, Inflammation, NFkB Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA. Phosphorylation at serine 536 stimulates acetylation at lysine 310 and interaction with the acetyltransferase CBP. The phosphorylated and acetylated forms show enhanced transcriptional activity.