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Anti-phospho p38MAPK (Thr180/Tyr182) Antibody
Catalog #: 130-10543
Rabbit Anti-Human phospho p38MAPK (Thr180/Tyr182) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q16539 |
| Gene Id | 1432 |
| Gene Symbols | MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A |
| Protein Name / Synonyms | Mitogen-activated protein kinase 14 (MAP kinase 14) (MAPK 14) (EC 2.7.11.24) (Cytokine suppressive anti-inflammatory drug-binding protein) (CSAID-binding protein) (CSBP) (MAP kinase MXI2) (MAX-interacting protein 2) (Mitogen-activated protein kinase p38 alpha) (MAP kinase p38 alpha) (Stress-activated protein kinase 2a) (SAPK2a) |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen phospho p38MAPK (Thr180/Tyr182) (176~186aa ). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:5, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
p38 MAP kinase (MAPK), also called RK or CSBP , is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress. Four isoforms of p38 MAPK, p38 (also known as Erk6 or SAPK3), and (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors . MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27. SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases .
Typical Data
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.