Anti-Phospho-p53-S9 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04637
Gene Id	7157
Gene Symbols	TP53
Protein Name / Synonyms	TP53, BCC7, LFS1, P53, TRP53, tumor protein p53
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Research Area	Apoptosis, Cancer, p53 Signaling, Autophagy, DNA Damage
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

23-43kDa

Observed MW

55kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S9 of human P53

Modification

Phospho S9

Storage

-20°C

Images

Western blot analysis of extracts of 293 and HT-29 cells, using Phospho-p53-S9 antibody (144-50085) at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. HT-29 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3min.
Western blot - Phospho-p53-S9 pAb (144-50085)

Immunofluorescence analysis of U2OS cells using Phospho-p53-S9 antibody (144-50085). Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-p53-S9 pAb (144-50085)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-p53-S9 pAb (144-50085). Western blot was performed from the immunoprecipitate using Phospho-p53-S9 pAb (144-50085) at a dilition of 1:1000. 293T cells were treated by UV at room temperature for 30 minutes after serum-starvation overnight, and then treated by 10% FBS at 37? for 30 minutes.
Immunoprecipitation - Phospho-p53-S9 pAb (144-50085)

Expiration:

12 months from the date of shipment when stored properly.

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