Anti-Phospho-PDGFRA-Y1018 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P16234
Gene Id	5156
Gene Symbols	PDGFRA
Protein Name / Synonyms	PDGFRA, CD140A, PDGFR-2, PDGFR2, GAS9, RHEPDGFRA, platelet-derived growth factor receptor alpha
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

24kDa/82kDa/122kDa

Observed MW

200kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding Y1018 of human PDGFRA

Modification

Phospho Y1018

Storage

-20°C

Images

Western blot analysis of extracts of HepG2 cells, using Phospho-PDGFRA-Y1018 antibody (144-50615) at 1:1000 dilution. HepG2 cells were treated by UV for 15-30 minutes or treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-PDGFRA-Y1018 antibody (144-50615)

Expiration:

12 months from the date of shipment when stored properly.

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