Phospho-PKC?/?-S643/676 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q04759|Q05655 |
| Gene Id | 5588|5580 |
| Gene Symbols | PRKCD, PRKCQ |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | PKC Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
77kDa/80kDa/67kDa/74kDa/81kDa
Observed MW
78kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S643/676 of human PKC?/?
Modification
Phospho S643/676
Storage
-20°C
Images
Western blot analysis of extracts of RAW264.7 cells, using Phospho-PKC?/?-S643/676 antibody (144-50567) at 1:1000 dilution. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.