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Anti-Phospho-RAF1-S259 Antibody
Catalog #: 144-50497
Phospho-RAF1-S259 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04049 |
| Gene Id | 5894 |
| Gene Symbols | RAF1 |
| Protein Name / Synonyms | RAF1, CMD1NN, CRAF, NS5, Raf-1, c-Raf, Raf-1 proto-oncogene, serine/threonine kinase |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
73kDa/75kDa
Observed MW
73kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S259 of human RAF1
Modification
Phospho S259
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using Phospho-RAF1-S259 antibody (144-50497) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-RAF1-S259 pAb (144-50497). Western blot was performed from the immunoprecipitate using Phospho-RAF1-S259 pAb (144-50497) at a dilition of 1:1000. HeLa cells were treated by 10% FBS at 37? for 30 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.