Anti-Phospho-Rb-S811 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P06400
Gene Id	5925
Gene Symbols	RB1
Protein Name / Synonyms	RB1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110, RB transcriptional corepressor 1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Dilutions

WB 1:1000 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

106kDa

Observed MW

106kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding S811 of human RB

Modification

Phospho S811

Storage

-20°C

Images

Western blot analysis of extracts of 293 and HeLa cells, using Phospho-Rb-S811 antibody (144-50089) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-Rb-S811 antibody (144-50089)

Immunofluorescence analysis of U2OS cells using Phospho-Rb-S811 antibody (144-50089). Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-Rb-S811 antibody (144-50089)

Expiration:

12 months from the date of shipment when stored properly.

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