Anti-Phospho-RELA-S311 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q04206
Gene Id	5970
Gene Symbols	RELA
Protein Name / Synonyms	RELA, NFKB3, p65, transcription factor p65
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

58kDa/59kDa/60kDa

Observed MW

60kDa

Immunogen

A synthetic phosphorylated peptide around S311 of human RELA (NP_068810.3).

Modification

Phospho S311

Storage

-20°C

Images

Western blot analysis of extracts of HeLa cells, using Phospho-RELA-S311 antibody (144-50445) at 1:1000 dilution or RELA antibody (144-11201). HeLa cells were treated by IFN-? (100 ng/ml) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - Phospho-RELA-S311 pAb (144-50445)

Expiration:

12 months from the date of shipment when stored properly.

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