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Anti-Phospho-RPS6KB1-S240/244 Antibody
Catalog #: 144-50537
Phospho-RPS6KB1-S240/244 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P62753 |
| Gene Id | 6194 |
| Gene Symbols | RPS6 |
| Protein Name / Synonyms | RPS6, S6, ribosomal protein S6 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
28kDa
Observed MW
36kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S240/244 of human RPS6KB1
Modification
Phospho S240/244
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-RPS6KB1-S240/244 antibody (144-50537) at 1:1000 dilution or RPS6 antibody. HeLa cells were treated by 10% FBS at 37? for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-RPS6-S240/244 pAb (144-50537). Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 pAb (144-50537) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37? for 30 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.